There are best chromatographs in world, most modern technological elaborations, but there is not one, which provides maximum efficiency of chromatographic column.

I have a lot of secrets in field of chromatography! One of them is attainment of maximum possible efficiency of chromatographic column (HAYRAPETYAN'S Effect). It is provided with new basic technology of chromatography - CHROMABAROGRAPHY (Russia patent "Chromatograph of A. S. Hayrapetyan").

The essence of new basic technology of chromatography - chromabarography is provision of optimal conditions of analysis by keeping unchanged linear rate of an imaginary point - zone of sample moved in time by carrier-gas from inlet to outlet of chromatographic column, ∆ p being kept constant at its ends during whole cycle of analysis, by which a maximum column efficiency (Hayrapetyan's Effect) is attained.

What is Chromabarography? The answer may be so: Or . . . so: The main advantages of proposed technology in practical analysis may be generated in form of following propositions:

Attainment of maximum possible efficiency of column on account of providing optimal conditions of analysis by keeping unchanged linear rate of an imaginary point - zone of sample moved in time by carrier-gas from inlet to outlet of chromatographic column;

Reduction of analysis time for a wide range of mixtures, which allows to work with a shorter column (economy of solid support, liquid phase, column material) and at much lower temperatures (economy of electricity) for which relative retention is generally high. Thus best separation is attained without increasing analysis time. This means an increase in productivity of routine analyses and elaboration of chromatographic methods;

"It's not difficult to know, it is difficult to get familiar". Skovoroda The theory of gas chromatography, presented by equation of Van Deemter,

(1) shows that for each chromatographic separation there exists an optimal flow rate above and below which column efficiency is reduced.

In practice, linear speed of sample zone moved by carrier gas, is changed continuously and increases as it approaches outlet, which results in a non-effective use of a part of column.

In this case equation (1) characterizes optimal separation process only in that section of chromatographic column through which sample passes at an optional speed.

In equation (1) are constant values and is speed of moving zone of sample which changes continuously in time. Thus equation (1) gets following form:

(2) where is speed of sample at a distance from beginning of column and changes - increases - toward outlet. This shows that HETP is not always optimal in all sections of chromatographic column. Let be length of chromatographic column, pressure at inlet of column and pressure at outlet of column, distance of a point on chromatographic column from its inlet. It is evident that pressure at point on column can be determined from correlation

The flow rate at any point on chromatographic column depends on pressure at that point, as well as on inlet pressure and outlet pressure of column. But pressure at any point on column depends on inlet pressure and outlet pressure of chromatographic column and on distance from column inlet. Thus flow rate at any point on column in case of fixed physical parameters of chromatographic column can be considered as a function of pressure at this point and at ends and of distance of this point from column inlet;

Consequently whole process in chromatographic column is characterized by a multiple of equations (3).

When at inlet and outlet of column pressure is kept constant, each section of chromatographic column, through which sample zone moves, is characterized by its equation from multiple (3).

However linear speed of sample zone moving with carrier-gas can be kept constant by programming pressure gradient movement along column in time, realizing function: pressure - location - time by keeping constant pressure difference ∆ p at ends of chromatographic column during whole cycle of analysis (Russia Patent "Chromatograph of A. S. Hayrapetyan").

The sample travels through chromatographic column in time

where is length of chromatographic column and optimal speed of sample zone.

The pressure at inlet and outlet of chromatographic column, undergoing a change at equal intervals of time, is expressed by

where - sample injection time, and - elution time.

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