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The advantage of establishing our page width in percentage terms, as 100%, is that page will adjust itself to resolution of screen. This is called a "liquid page design" (although I like to call it a "bubble-gum page design", since page looks as if it has been stretched horizontally when viewed at higher resolutions than intended). The main benefit of this method is that all available screen real estate will be utilized. The disadvantage is that layout of your page will change, and users may find it uncomfortable to read extremely wide sentences that will result when higher resolutions are used.
If you decide follow our advice to optimize your page for 800x600, and then decide to follow fixed-width page method, you must take into account that not all 800 pixels will be available (some of them will be used by browser to display scrollbar and other "browser chrome"). To accommodate most browsers and platforms, specify your page width at a maximum of 750 pixels.
If you decide to specify your page at 100% width, you won't have that problem, since it will adjust automatically to available width. Just make sure that your page looks best at 800x600, and that layout doesn't change much when you view your page at 1024x768.
Mario Sanchez publishes The Internet Digest ( http://www.theinternetdigest.net ), an online collection of web design and Internet marketing articles and resources. You can freely reprint his weekly articles in your website, ezine, newsletter or ebook.